মঙ্গলবার, ৩ জুলাই, ২০১২

Four foreign aid workers rescued in Somalia

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Source: http://news.yahoo.com/four-foreign-aid-workers-rescued-somalia-army-062948312.html

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Researchers improve living tissues with 3-D printed vascular networks made from sugar

Researchers improve living tissues with 3-D printed vascular networks made from sugar

Monday, July 2, 2012

Researchers are hopeful that new advances in tissue engineering and regenerative medicine could one day make a replacement liver from a patient's own cells, or animal muscle tissue that could be cut into steaks without ever being inside a cow. Bioengineers can already make 2D structures out of many kinds of tissue, but one of the major roadblocks to making the jump to 3D is keeping the cells within large structures from suffocating; organs have complicated 3D blood vessel networks that are still impossible to recreate in the laboratory.

Now, University of Pennsylvania researchers have developed an innovative solution to this perfusion problem: they've shown that 3D printed templates of filament networks can be used to rapidly create vasculature and improve the function of engineered living tissues.

The research was conducted by a team led by postdoctoral fellow Jordan S. Miller and Christopher S. Chen, the Skirkanich Professor of Innovation in the Department of Bioengineering at Penn, along with Sangeeta N. Bhatia, Wilson Professor at the Massachusetts Institute of Technology, and postdoctoral fellow Kelly R. Stevens in Bhatia's laboratory.

Their work was published in the journal Nature Materials.

Without a vascular system ? a highway for delivering nutrients and removing waste products ? living cells on the inside of a 3D tissue structure quickly die. Thin tissues grown from a few layers of cells don't have this problem, as all of the cells have direct access to nutrients and oxygen. Bioengineers have therefore explored 3D printing as a way to prototype tissues containing large volumes of living cells.

The most commonly explored techniques are layer-by-layer fabrication, or bioprinting, where single layers or droplets of cells and gel are created and then assembled together one drop at a time, somewhat like building a stack of LEGOs.

Such "additive manufacturing" methods can make complex shapes out of a variety of materials, but vasculature remains a major challenge when printing with cells. Hollow channels made in this way have structural seams running between the layers, and the pressure of fluid pumping through them can push the seams apart. More important, many potentially useful cell types, like liver cells, cannot readily survive the rigors of direct 3D bioprinting.

To get around this problem, Penn researchers turned the printing process inside out.

Rather than trying to print a large volume of tissue and leave hollow channels for vasculature in a layer-by-layer approach, Chen and colleagues focused on the vasculature first and designed free-standing 3D filament networks in the shape of a vascular system that sat inside a mold. As in lost-wax casting, a technique that has been used to make sculptures for thousands of years, the team's approach allowed for the mold and vascular template to be removed once the cells were added and formed a solid tissue enveloping the filaments.

"Sometimes the simplest solutions come from going back to basics," Miller said. "I got the first hint at this solution when I visited a Body Worlds exhibit, where you can see plastic casts of free-standing, whole organ vasculature."

This rapid casting technique hinged on the researchers developing a material that is rigid enough to exist as a 3D network of cylindrical filaments but which can also easily dissolve in water without toxic effects on cells. They also needed to make the material compatible with a 3D printer so they could make reproducible vascular networks orders of magnitude faster, and at larger scale and higher complexity, than possible in a layer-by-layer bioprinting approach.

After much testing, the team found the perfect mix of material properties in a humble material: sugar. Sugars are mechanically strong and make up the majority of organic biomass on the planet in the form of cellulose, but their building blocks are also typically added and dissolved into nutrient media that help cells grow.

"We tested many different sugar formulations until we were able to optimize all of these characteristics together," Miller said. "Since there's no single type of gel that's going to be optimal for every kind of engineered tissue, we also wanted to develop a sugar formula that would be broadly compatible with any cell type or water-based gel."

The formula they settled on ? a combination of sucrose and glucose along with dextran for structural reinforcement ? is printed with a RepRap, an open-source 3D printer with a custom-designed extruder and controlling software. An important step in stabilizing the sugar after printing, templates are coated in a thin layer of a degradable polymer derived from corn. This coating allows the sugar template to be dissolved and to flow out of the gel through the channels they create without inhibiting the solidification of the gel or damaging the growing cells nearby. Once the sugar is removed, the researchers start flowing fluid through the vascular architecture and cells begin to receive nutrients and oxygen similar to the exchange that naturally happens in the body.

The whole process is quick and inexpensive, allowing the researchers to switch with ease between computer simulations and physical models of multiple vascular configurations.

"This new platform technology, from the cell's perspective, makes tissue formation a gentle and quick journey," Chen said, "because cells are only exposed to a few minutes of manual pipetting and a single step of being poured into the molds before getting nourished by our vascular network."

The researchers showed that human blood vessel cells injected throughout the vascular networks spontaneously generated new capillary sprouts to increase the network's reach, much in the way blood vessels in the body naturally grow. The team then created gels containing primary liver cells to test whether their technique could improve their function.

When the researchers pumped nutrient-rich media through the gel's template-fashioned vascular system, the entrapped liver cells boosted their production of albumin and urea, natural components of blood and urine, respectively, which are important measures of liver-cell function and health. There was also clear evidence of increased cell survival around the perfused vascular channels.

And theoretical modeling of nutrient transport in these perfused gels showed a striking resemblance to observed cell-survival patterns, opening up the possibility of using live-cell data to refine computer models to better design vascular architectures.

Though these engineered tissues were not equivalent to a fully functioning liver, the researchers used cell densities that approached clinical relevance, suggesting that their printed vascular system could eventually be used to further research in lab-grown organs and organoids.

"The therapeutic window for human-liver therapy is estimated at one to 10 billion functional liver cells," Bhatia said. "With this work, we've brought engineered liver tissues orders of magnitude closer to that goal, but at tens of millions of liver cells per gel we've still got a ways to go.

"More work will be needed to learn how to directly connect these types of vascular networks to natural blood vessels while at the same time investigating fundamental interactions between the liver cells and the patterned vasculature. It's an exciting future ahead."

With promising indications that their vascular networks will be compatible with all types of cells and gels, the team believes their 3D printing method will be a scalable solution for a wide variety of cell- and tissue-based applications because all organ vasculature follows similar architectural patterns.

"Cell biologists like the idea of 3D printing to make vascularized tissues in principle, but they would need to have an expert in house and highly specialized equipment to even attempt it," Miller said. "That's no longer the case; we've made these sugar-based vascular templates stable enough to ship to labs around the world."

Beyond integrating well with the world of tissue engineering, the researchers' work epitomizes the philosophy that drives much of the open source 3D printing community.

"We launched this project from innovations rooted in RepRap and MakerBot technology and their supporting worldwide communities," Miller said. "A RepRap 3D printer is a tiny fraction of the cost of commercial 3D printers, and, more important, its open-source nature means you can freely modify it. Many of our additions to the project are already in the wild."

Several of the custom parts of the RepRap printer the researchers used to make the vascular templates were printed in plastic on another RepRap. Miller will teach a class on building and using these types of printers at a workshop this summer and will continue tinkering with his own designs.

"We want to redesign the printer from scratch and focus it entirely on cell biology, tissue engineering and regenerative medicine applications," Miller said.

###

University of Pennsylvania: http://www.upenn.edu/pennnews

Thanks to University of Pennsylvania for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

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Source: http://www.labspaces.net/121396/Researchers_improve_living_tissues_with___D_printed_vascular_networks_made_from_sugar

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সোমবার, ২ জুলাই, ২০১২

The states that are dying for healthcare coverage

By Alexander E.M. Hess and Samuel Weigley, 24/7 Wall St.

The lack of medical coverage in America is a serious problem as approximately 50 million people were uninsured all through 2010. But the U.S. Supreme Court on Thursday ruled the Patient Protection and Affordable Care Act, which was passed in 2010, was constitutional. The legislation, once implemented in its entirety, is expected to cover 30 million Americans currently lacking coverage.

The lack of medical insurance has had grave consequences for individuals and the nation. In 2010 alone, 26,100 people died because they had no health insurance ? that amounts to 502 preventable deaths a week. However, some states fared better than others. Based on the latest report by Families USA, a health care consumer advocacy group, 24/7 Wall St. identified the 10 states with the highest number of deaths per 100,000 people due to a lack of insurance.

24/7 Wall St.: Eight states slashing local funding

Not surprisingly, nearly all of the states with the most residents dying due to a lack of insurance also had high numbers of uninsured residents. Seven of the states on the list were among the 10 states with the highest percentage of people without health coverage. Seven of the states were also in the bottom 10 for the lowest rates of private insurance coverage.

People without health insurance often forgo medical treatment for different reasons. According to Families USA, a supporter of President Obama?s health care reform law, uninsured adults are nearly four times more likely than insured adults to delay or avoid preventive care screening due to cost. Uninsured adults are also nearly seven times more likely to go without needed care due to cost than privately insured adults.

?You still see a very, very strong correlation between uninsurance and poor health-care outcomes ? including mortality ? and [that is] because people aren?t getting the type of care that they need,? Kim Bailey, the research director for Families USA, told 24/7 Wall St.

Many of the states with high death rates due to a lack of insurance also were among the poorest states in the country. The top seven states on this list also are among the 10 states with the highest poverty rates. Every state on this list is in the top half.

Poor health also appears to play an important role. States with high death rates due to lack of insurance had a high percentage of people with lifestyle-related risk factors for poor health. Of the states on our list, five of them have among the 10 highest percentages of smokers and among the 10 lowest percentages of people who eat vegetables at least three times a day. Four have among the 10 highest proportions of overweight or obese adults. Seven states on the list were in the bottom 10 in terms of life expectancy.

24/7 Wall St.: Countries that spend the most on health care

Based on Families USA?s report, ?Dying for Coverage: The Deadly Consequences of Being Uninsured,? 24/7 Wall St. identified the 10 states with the highest number of deaths from being uninsured per 100,000 residents. 24/7 Wall St. reviewed the methodology used by Families USA, first developed in 2002 by the Institute of Medicine, to determine?excess mortality from being uninsured. This method considers the proportion of?people who are insured and uninsured, the mortality risks for the uninsured and the number of expected deaths from a hypothetical fully insured population. 24/7 Wall St. also identified poverty rates and median income by state, provided by the U.S. Census Bureau. The Kaiser Family Foundation?s website ? Statehealthfacts.org ? provided health-related data, including life expectancy, obesity and diabetes rate.

These are the 10 states dying for health coverage.

1. Mississippi
? Excess deaths from a lack of insurance (per 100,000):?15.82
? Percent of population uninsured:?18.2 percent (ninth highest)
? Percent living below the poverty line:?22.4 percent (tied for the highest)
? Life expectancy at birth:?74.81 years (The lowest)

Many residents of Mississippi cannot afford insurance. The state has the lowest median income in the nation and the highest percentage of residents living below the poverty line. As a result, Mississippi has the second-lowest percentage of residents with private health insurance coverage, at 56.49 percent. Exacerbating the problem, residents are especially unhealthy. Among all states, Mississippi has the second-highest obesity rate, the second-highest percentage of adults with diabetes and the fifth-highest percentage of adult smokers in the nation. Probably on account of both high uninsurance rates and poor personal health, Mississippi is the only state where life expectancy was below 75 years at birth in 2010. Mississippi?s excess death rate was the highest among all states and twice that of 28 states in 2010.

2. Louisiana
? Excess deaths from a lack of insurance (per 100,000):?14.94
? Percent of population uninsured:?17.8 percent (10th highest)
? Percent living below the poverty line:?18.7 percent (sixth highest)
? Life expectancy at birth:?75.39 years (fourth lowest)

Louisiana has one of the lowest life expectancies at birth in the U.S. at 75.4 years. Though much of this certainly can be attributed to poor health choices ? the state has a higher number of smokers and its residents eat comparatively little fruit or vegetables ? the inability of?many residents to receive proper care due to lack of insurance is also a contributing factor. In Louisiana, 17.8 percent of the population goes without health insurance, despite the fact that 21.9 percent of the population qualifies for Medicaid ? the fifth-highest proportion among all 50 states. The high uninsurance rate is partly due to the relative economic disadvantage of the state?s residents. With 18.7 percent of residents living below the poverty line ? the sixth-highest rate in the nation ? and a median income that is more than $5,000 lower than the U.S. average, just 58.39 percent of state residents have private insurance. That is the fourth-lowest such rate in the nation.

3. Arkansas
? Excess deaths from a lack of insurance (per 100,000):?13.49
? Percent of population uninsured:?17.5 percent (tied for 12th highest)
? Percent living below the poverty line:?18.8 percent (fifth highest)
? Life expectancy at birth:?76.09 years (sixth lowest)

According to the Council for Community and Economic Research?s ACCRA Cost of Living Index, Arkansas had the second-lowest cost of health care in the United States. However, with 18.8 percent of the population living below the poverty line and a median annual household income of just $38,307 ? both among the lowest figures for any state ? many Arkansans cannot afford private health coverage. As a result, just 58.78 percent of the population has private insurance, the sixth-lowest figure in the country.

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4. South Carolina
? Excess deaths from a lack of insurance (per 100,000):?13.48
? Percent of population uninsured:?17.5 percent (tied for 12th highest)
? Percent living below the poverty line:?18.2 percent (seventh highest)
? Life expectancy at birth:?76.57 years (ninth lowest)

South Carolina is not a particularly healthy state: 67.4 percent of the state?s residents are either overweight or obese, just 23.3 percent eat proper amounts of fruit, only 22.9 percent eat proper amounts of vegetables and 10.7 percent are diabetic. All of these are among the highest rates in the country. Meanwhile, much of the cost of health care falls to private individuals. The state spent about $6,300 per person on health care in 2009, among the lowest levels, and just 51.9 percent of residents have employer-based health coverage. Unfortunately, South Carolinians have trouble affording insurance on their own: Median income was just $42,000 in 2010, significantly lower than the $50,000 national average, 18.2 percent of residents live below the poverty line and the cost of health care is higher than is the case in many states.

5. New Mexico
? Excess deaths from a lack of insurance (per 100,000):?12.15
? Percent of population uninsured:?19.6 percent (sixth highest)
? Percent living below the poverty line:?20.4 percent (tied for the highest)
? Life expectancy at birth:?78.21 years (20th lowest)

New Mexico has a fairly healthy population, with relatively low heart disease and obesity rates. However, just 55.8 percent of residents have private health insurance ? the lowest rate of any state in the country. One possible reason is that few employers provide insurance ? just 45.6 percent of the population has employer-based health coverage. The relative poverty of the state also means many residents cannot afford medical coverage. The median income in the state was just above $42,000 in 2010, far below the national median of about $50,000, while 20.4 percent of people live below the poverty line ? the highest rate in the country.

Click here to read the rest of 24/7 Wall St.'s analysis of states' death rates

More money and business news:

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Source: http://bottomline.msnbc.msn.com/_news/2012/07/01/12419471-the-states-that-are-dying-for-healthcare-coverage?lite

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RIM Is Hurting, But It?s Not Dead Just Yet

rim-5There?s been plenty said about RIM since it released its rough fiscal financials a few days ago, just about all of it negative. It?s hard not to be, honestly ? the company reported its first operating loss in eight years, announced that a full 5,000 employees would be getting the axe, and topped it all off by revealing that the first BlackBerry 10 devices wouldn?t hit the market until the beginning of 2013. It seems to me that RIM's problem (well, one of them anyway) is that the company's brass seems to look at the game they?re playing as one of time, and they think they have plenty of it.

Source: http://feedproxy.google.com/~r/Techcrunch/~3/rN0Gw1LW6OE/

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রবিবার, ১ জুলাই, ২০১২

Morsi sworn in as Egypt's first civilian president

Mohamed Morsi was sworn in as Egypt's first freely elected civilian president on Saturday and formally received a transfer of power and pledge of support from the military, which has ruled since last year.

"I swear by the Almighty God to sincerely preserve the republican order and to respect the constitution and law, and completely care for the people's interest," said the 60-year-old Morsi in a ceremony at the constitutional court.

The swearing-in, aired lived on national television, was delayed because of a dispute between Morsi, who did not want it broadcast, and judges, who insisted on it, one of the judges told the state's Al-Ahram newspaper.

Morsi had also wanted to take the oath before the Islamist-led parliament, but the military dissolved it earlier this month following a court order in what the Muslim Brotherhood described as a "soft coup."

In the handover at Cairo's Hike Step base, Field Marshal Hussein Tantawi pledged to support Morsi, who had been a senior Brotherhood figure until resigning from the movement after his election and with whom the army has had uneasy relations.

"We will stand with the new president, elected by the people," the head of the Supreme Council of the Armed Forces (SCAF) said in a speech after an honour guard parade and a helicopter fly-past.

He saluted Morsi and awarded him the highest military honour, "the shield of the armed forces."

Morsi thanked the military and pledged to support it.

"I accept the transfer of power," he said in his speech, at the same base where members of the once-banned Brotherhood had faced military trials under Mubarak.

Just after taking the oath of office, Morsi went to Cairo University to deliver a speech in which he pointedly mentioned the "elected parliament" several times and said the army should resume its normal role.

"The elected institutions will return to fulfilling their roles. And the great military will devote itself to the task of protecting the country," he said.

He then set out some of his international and domestic objectives, saying he would be a "servant of the people" in a "democratic, modern and constitutional state".

Internationally, he said Egypt respected would back the Palestinians and called for an end to the bloodshed in Syria.

"I announce from here that Egypt, its people and presidential institution stand with the Palestinian people until they regain all their rights," he said.

"We support the Syrian people. We want the bloodshed to stop," he added.

He repeated that Egypt would respect its international treaties, in an allusion to its 1979 peace accord with Israel.

Morsi had spoken out forcefully in support of Palestinians during his campaign.

The Brotherhood is vehemently opposed to Syrian president Bashar al-Assad and supports the uprising against him.

But as president, Morsi is not expected to radically change his country's foreign policy, especially towards Israel, in which the military is expected to exercise its clout.

In a Friday speech before tens of thousands of supporters in Cairo's Tahrir Square, the epicentre of the revolt that ousted Mubarak, he said that he would insist on retaining all the presidency's powers.

"I renounce none of the prerogatives of president," he told his supporters, adding: "You are the source of power and legitimacy."

"There is no place for anyone or any institution ... above this will."

The SCAF assumed legislative powers after disbanding parliament and also formed a powerful national security council headed by the president but dominated by the generals.

The military also reserves the right to appoint a new constituent assembly should the one elected by parliament be disbanded by a court decision expected on September 1.

The Muslim Brotherhood insists that only parliament can appoint the assembly.

Media reports said Morsi was consulting a cross-section of Egyptian society before appointing a premier and a cabinet made up mostly of technocrats.

In a meeting with newspaper editors reported by most dailies on Friday, he pledged there would be "no Islamisation of state institutions" during his presidency.

Morsi became the Brotherhood's candidate to succeed Mubarak only after its first choice, Khairat El-Shater, was disqualified. He beat Ahmed Shafiq, Mubarak's last premier, with 51.73 percent of the vote.

Many had written him off as an uncharismatic substitute, saying he would be unable to muster widespread support.

But the powerful Brotherhood mobilised its formidable resources and supporters behind Morsi, who was appointed last year to head its political arm, the Freedom and Justice Party.

Source: http://news.yahoo.com/egypts-morsi-tahrir-tribute-oath-051252331.html

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Earn Money With A Social Networking Script And Video Marketing ...

Jun 30 2012

Social networking sites are big business so imagine the money you could earn if you had your own social network site which you can also use for video marketing. To create your own site you could use a script designed for social networking sites like Phpfox. Let?s take a closer look at how you can benefit from Phpfox.

Phpfox is undoubtedly an incredible social network script which will allow any site owner to make their own social networking site in any niche they want. There are new social networking sites coming up all over the internet due to the great popularity of websites like Facebook and Twitter. The income potential to having your own social network site is extremely high so now a lot of webmasters are making their very own sites.

Consider the advantages which come with having your very own social networking website: You do not have to continuously add fresh content to your website because it?s continuously being added by your members; As your members invite colleagues and friends to sign up, they?re promoting your own website for you; When newbies complete their profiles they?re providing you all your marketing research as well as market details.

Using the Phpfox script you can make your own social networking site with all of the essential functions a whole bunch more. Of course when studying the various scripts available you need to read reviews from various other website owners, programmers as well as developers so that you can learn from other people whether the script provides you with the things you are searching for.

The Phpfox also offers a forum of members who are also utilizing the scripts so you can get a lot of insight when needed. With scripts and creating websites like social networking sites, a member?s forum can be very helpful. With forums like this it?s easy to find information and facts you simply don?t get anywhere else and even any changes or tips that can help you to utilize the script better and get the most from it.

A lot of social network scripts can be hard to install and implement the numerous options but the phpfox script is among the simplest scripts available. The installation process is conducted in three basic steps which are fairly easy. 1. Make a database; 2. Add the script files then chmod them depending on the directories; 3. Go to the installation page and type in your details.

The information required is the database name, your username and your password. That is it, you then just run it and the installation file does the rest. If you are not comfortable installing the script yourself you can have the Phpfox Company install it for you for a small fee. When the script is installed you can then log yourself into the admin area and choose the template you want for your social networking website. There are a number of templates to choose from with the phpfox script.

If you?re considering making your own social networking website for video marketing then Phpfox is unquestionably one of the top scripts to do it with. However you need to explore all of your options and select which is most effective for you.

Source: http://www.aboutmoray.com/earn-money-with-a-social-networking-script-and-video-marketing

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